DNA Vectors
BIOHIPPO AAV-RAM-d2tTA-TRE-FLEX-hM4D-mCherry-WPREs
Recombinant adeno associated virus expressing d2TTA hM4D fused to mCherry under the RAM TRE promoter with WPRE and hGH polyA elements. The Tet responsive configuration enables doxycycline controlled expression, providing precise chemogenetic control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Vector Biolabs AAV-CAG-GFP (AAV serotype 4) AAV (AAV4-CAG-EGFP)
The CAG promoter is a hybrid of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter. The CAG promoter drives high levels of gene expression in mammalian expression vectors than CMV promoter alone.This AAV serotype 4 virus (Capsid from AAV-4 and ITR from AAV2) expresses GFP (Green Fluorescent Protein) under the control of CAG promoter.
Vector Biolabs AAV-CAG-GFP (AAV serotype 8-DJ) AAV (AAV8/DJ-CAG-eGFP)
This AAV-8 (DJ) virus expresses eGFP under a CAG/CBA promoter.
Vector Biolabs Lymphoid enhancer-binding factor 1 Adenovirus (Ad-LEF1)
T-cell factor (TCF-1) and lymphoid enhancer-binding factor (for LEF-1, also designated TCF-1(alpha)) compose a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. LEF-1, TCF-1, and two other family members, TCF-3 and TCF-4, directly bind to cytosolic beta-catenin and facilitate its translocation to the nucleus, where these complexes induce expression of Wnt target genes.
Vector Biolabs AAV-iCre (AAV5) AAV (AAV5-iCre)
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.This AAV5 stock expresses an improved Cre (iCre) under control of a CMV promoter.
Vector Biolabs AAV-EGFP-iCre (AAV9) AAV (AAV9-EGFP-iCre)
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.This AAV-DJ/9 stock expresses an improved Cre (iCre) and an eGFP driven by one CMV promoter. The iCre and eGFP was separated by a 2A peptides.
BIOHIPPO AAV-PV-EGFP-5'miR-30-shRNA(scramble)-3'miR-30-WPREs
Recombinant adeno associated virus expressing 5'miR-30-shRNA(scramble)-3'miR-30 fused to EGFP under the PV promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-VGAT1-DIO-DTA-2A-EGFP-WPRE-hGH-polyA
Recombinant adeno associated virus expressing DTA fused to EGFP under the VGAT1 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-EF1a-DIO-EGFP-EGFP-T2A-TVA-WPRE-hGH-polyA
Recombinant adeno associated virus expressing TVA fused to EGFP under the EF1a promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-CaMKIIa-DIO-DTA-2A-EGFP-WPRE-hGH聽-polyA
Recombinant adeno associated virus expressing DTA fused to EGFP under the CaMKIIa promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Vector Biolabs Adiponectin receptor 2 Adenovirus (Ad-Adipo-R2)
The adiponectin receptors, ADIPOR1 and ADIPOR2, serve as receptors for globular and full-length adiponectin and mediate increased AMPK and PPAR-alpha (PPARA) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin
IBA LifeSciences pASK-IBA3C
pASK-IBA3C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the C-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), and the inducible tetracycline promoter/operator for the regulated expression of proteins.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. The recombinant protein will be localized in the cytoplasm.
Vector Biolabs Sus scrofa GNAS shRNA Adenovirus (Ad-S-GNAS-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown Sus scrofa GNAS gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this Sus scrofa gene was validated by western blot in IPI21 cells.